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Functional characterisation of human synaptic genes expressed in the Drosophila brain

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Item Type:Article
Title:Functional characterisation of human synaptic genes expressed in the Drosophila brain
Creators Name:Zografos, L. and Tang, J. and Hesse, F. and Wanker, E.E. and Li, K.W. and Smit, A.B. and Davies, R.W. and Armstrong, J.D.
Abstract:Drosophila melanogasteris an established and versatile model organism. Here we describe and make available a collection of transgenicDrosophilastrains expressing human synaptic genes. The collection can be used to study and characterise human synaptic genes and their interactions and as controls for mutant studies. It was generated in a way that allows the easy addition of new strains, as well as their combination. In order to highlight the potential value of the collection for the characterisation of human synaptic genes we also use two assays, investigating any gain-of-function motor and/or cognitive phenotypes in the strains in this collection. Using these assays we show that among the strains made there are both types of gain-of-function phenotypes investigated. As an example, we focus on the three strains expressing human tyrosine protein kinase Fyn, the small GTPase Rap1a and human Arc, respectively. Of the three, the first shows a cognitive gain-of-function phenotype while the second a motor gain-of-function phenotype. By contrast, Arc, which has noDrosophilaortholog, shows no gain-of-function phenotype.
Keywords:Fruit Fly, Human, Synaptic, Synapse, Synaptic Proteome, Synaptopathy, Mental Illness, Characterisation, Courtship, Climbing, Animals, Drosophila Melanogaster
Source:Biology Open
Publisher:Company of Biologists
Page Range:662-667
Date:15 May 2016
Official Publication:https://doi.org/10.1242/bio.016261
PubMed:View item in PubMed

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