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Testing for antibodies to human aquaporin-4 by ELISA: sensitivity, specificity, and direct comparison with immunohistochemistry

Item Type:Article
Title:Testing for antibodies to human aquaporin-4 by ELISA: sensitivity, specificity, and direct comparison with immunohistochemistry
Creators Name:Jarius, S. and Franciotta, D. and Paul, F. and Bergamaschi, R. and Rommer, P.S. and Ruprecht, K. and Ringelstein, M. and Aktas, O. and Kristoferitsch, W. and Wildemann, B.
Abstract:Background: Several assays have been developed to detect antibodies to aquaporin-4 (NMO-IgG/AQP4-Ab). However, many of these assays require sophisticated techniques and are thus only available at specialized laboratories. This is problematic since NMO-IgG/AQP4-Ab testing has important prognostic and therapeutic implications. Objective: To evaluate a newly developed, commercial, enzyme-linked immunosorbent assay (ELISA) for detecting NMO-IgG/AQP4-Ab. Methods: Serum samples from 261 patients with NMO spectrum disorders (NMOSD; n = 108) and controls (n = 153) were tested for AQP4-Ab by using ELISA. Of these patients, 207 were tested in parallel using a standard immunohistochemical (IHC) assay. Results: Fifty of 66 (75.8%) patients with NMO, 17/25 (68%) with LETM, 3/14 (21.4%) with ON, 2/3 (66.7%) with ON and non-extensive transverse myelitis, and 2/153 (1.3%) controls tested positive in the ELISA. Of those NMOSD patients tested by both ELISA and IHC, 10 were positive only in the ELISA and 3 exclusively in the IHC assay, suggesting that the overall sensitivity of the ELISA was higher than that of the standard IHC assay. The ELISA yielded very good intra- and inter-run reproducibility with regard to AQP4-Ab detection and good intrarun, but only moderate inter-run reproducibility with regard to AQP4-Ab quantification. Anti-AQP4 serum concentrations correlated with disease activity (p < 0.00001), but did not differ between patients with NMO and patients with isolated LETM or ON. Conclusion: The ELISA evaluated here provides a relatively sensitive and easy-to-use diagnostic tool for detecting antibodies to AQP4 and could make AQP4-Ab testing, which is of high clinical relevance, more widely available.
Keywords:Neuromyelitis Optica (Devic's Syndrome), NMO-IgG, Antibody to Aquaporin-4, Diagnosis, Recombinant Antigen, Enzyme-Linked Immunosorbent Assay (ELISA), Indirect Iimmunofluorescence, Longitudinally Extensive Transverse Myelitis, Optic Neuritis, Multiple Sclerosis
Source:Journal of the Neurological Sciences
ISSN:0022-510X
Publisher:Elsevier (The Netherlands)
Volume:320
Number:1-2
Page Range:32-37
Date:15 September 2012
Official Publication:https://doi.org/10.1016/j.jns.2012.06.002
PubMed:View item in PubMed

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