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An ex vivo model for studying mitochondrial trafficking in neurons

Item Type:Article
Title:An ex vivo model for studying mitochondrial trafficking in neurons
Creators Name:Bros, H. and Niesner, R. and Infante-Duarte, C.
Abstract:Distribution of mitochondria throughout the cytoplasm is necessary for cellular function and health. Due to their unique, highly polarized morphology, neurons are particularly vulnerable to defects of mitochondrial transport, and its disruption can contribute to neuropathology. In this chapter, we present an ex vivo method for monitoring mitochondrial transport within myelinated sensory and motor axons from spinal nerve roots. This approach can be used to investigate mitochondrial behavior under a number of experimental conditions, e.g., by applying ion channel modulators, ionophores, or toxins, as well as for testing the therapeutic potential of new strategies targeting axonal mitochondrial dynamics.
Keywords:Mitochondrial Transport, Mitochondrial Trafficking, Axonal Transport, Mitochondria Live Imaging, Spinal Nerve Roots, Ex Vivo Explants, Animals
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Mitochondrial Medicine
Publisher:Springer / Humana Press
Page Range:465-472
Official Publication:https://doi.org/10.1007/978-1-4939-2257-4_38
PubMed:View item in PubMed

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