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Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis

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Item Type:Article
Title:Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis
Creators Name:Metz, I. and Beißbarth, T. and Ellenberger, D. and Pache, F. and Stork, L. and Ringelstein, M. and Aktas, O. and Jarius, S. and Wildemann, B. and Dihazi, H. and Friede, T. and Brück, W. and Ruprecht, K. and Paul, F.
Abstract:Objective: To assess in an observational study whether serum peptide antibody reactivities may distinguish aquaporin-4 (AQP4) antibody (Ab)–positive and -negative neuromyelitis optica spectrum disorders (NMOSD) and relapsing-remitting multiple sclerosis (RRMS). Methods: We screened 8,700 peptides that included human and viral antigens of potential relevance for inflammatory demyelinating diseases and random peptides with pooled sera from different patient groups and healthy controls to set up a customized microarray with 700 peptides. With this microarray, we tested sera from 66 patients with AQP4-Ab-positive (n = 16) and AQP4-Ab-negative (n = 19) NMOSD, RRMS (n = 11), and healthy controls (n = 20). Results: Differential peptide reactivities distinguished NMOSD subgroups from RRMS in 80% of patients. However, the 2 NMOSD subgroups were not well-discriminated, although those patients are clearly separated by their antibody reactivities against AQP4 in cell-based assays. Elevated reactivities to myelin and Epstein-Barr virus peptides were present in RRMS and to AQP4 and AQP1 peptides in AQP4-Ab-positive NMOSD. Conclusions: While AQP4-Ab-positive and -negative NMOSD subgroups are not well-discriminated by peptide antibody reactivities, our findings suggest that peptide antibody reactivities may have the potential to distinguish between both NMOSD subgroups and MS. Future studies should thus concentrate on evaluating peptide antibody reactivities for the differentiation of AQP4-Ab-negative NMOSD and MS.
Keywords:Cell-Based Assay, Antigen Microarrays, Diagnostic-Criteria, Immunoglobulin-G, Spectrum, Antibodies, Targets, Lesions, Marker, IgG
Source:Neurology Neuroimmunology & Neuroinflammation
Publisher:American Academy of Neurology
Page Range:e204
Date:April 2016
Official Publication:https://doi.org/10.1212/NXI.0000000000000204
PubMed:View item in PubMed

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