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| Item Type: | Article |
|---|---|
| Title: | Synaptic network activity induces neuronal differentiation of adult hippocampal precursor cells through BDNF signaling |
| Creators Name: | Babu, H. and Ramirez-Rodriguez, G. and Fabel, K. and Bischofberger, J. and Kempermann, G. |
| Abstract: | Adult hippocampal neurogenesis is regulated by activity. But how do neural precursor cells in the hippocampus respond to surrounding network activity and translate increased neural activity into a developmental program? Here we show that long-term potentiation (LTP)-like synaptic activity within a cellular network of mature hippocampal neurons promotes neuronal differentiation of newly generated cells. In co-cultures of precursor cells with primary hippocampal neurons, LTP-like synaptic plasticity induced by addition of glycine in Mg(2+)-free media for 5 min, produced synchronous network activity and subsequently increased synaptic strength between neurons. Furthermore, this synchronous network activity led to a significant increase in neuronal differentiation from the co-cultured neural precursor cells. When applied directly to precursor cells, glycine- and Mg(2+)-free solution did not induce neuronal differentiation. Synaptic plasticity-induced neuronal differentiation of precursor cells was observed in the presence of GABAergic neurotransmission blockers but was dependent on NMDA-mediated Ca(2+) influx. Most importantly, neuronal differentiation required the release of brain-derived neurotrophic factor (BDNF) from the underlying substrate hippocampal neurons as well as TrkB receptor phosphorylation in precursor cells. This suggests that activity-dependent stem cell differentiation within the hippocampal network is mediated via synaptically evoked BDNF signaling. |
| Keywords: | Hippocampus, Precursor Cell, Adult Neurogenesis, Long-Term Potentiation, Neurotrophins, Network Oscillation |
| Source: | Frontiers in Neuroscience |
| ISSN: | 1662-453X |
| Publisher: | Frontiers Media SA |
| Volume: | 3 |
| Page Range: | 49 |
| Date: | 30 September 2009 |
| Official Publication: | https://doi.org/10.3389/neuro.22.001.2009 |
| PubMed: | View item in PubMed |
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