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PCNA appears in two populations of slow and fast diffusion with a constant ratio throughout S-phase in replicating mammalian cells

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Item Type:Article
Title:PCNA appears in two populations of slow and fast diffusion with a constant ratio throughout S-phase in replicating mammalian cells
Creators Name:Zessin, P.J.M. and Sporbert, A. and Heilemann, M.
Abstract:DNA replication is a fundamental cellular process that precedes cell division. Proliferating cell nuclear antigen (PCNA) is a central scaffold protein that orchestrates DNA replication by recruiting many factors essential for the replication machinery. We studied the mobility of PCNA in live mammalian cells using single-particle tracking in combination with photoactivated-localization microscopy (sptPALM) and found two populations. The first population which is only present in cells with active DNA replication, showed slow diffusion and was found to be located in replication foci. The second population showed fast diffusion, and represents the nucleoplasmic pool of unbound PCNA not involved in DNA replication. The ratio of these two populations remained constant throughout different stages of S-phase. A fraction of molecules in both populations showed spatially constrained mobility. We determined an exploration radius of ~100 nm for 13% of the slow-diffusing PCNA molecules, and of ~600 nm for 46% of the fast-diffusing PCNA molecules.
Keywords:Cell Division, Cell Line, DNA Replication, Diffusion, Molecular Imaging, Proliferating Cell Nuclear Antigen, Protein Transport, S Phase, Animals
Source:Scientific Reports
ISSN:2045-2322
Publisher:Nature Publishing Group (U.K.)
Volume:6
Page Range:18779
Date:13 January 2016
Official Publication:https://doi.org/10.1038/srep18779
PubMed:View item in PubMed

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