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Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections

Item Type:Article
Title:Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections
Creators Name:Cano, E. and Carmona, R. and Ruiz-Villalba, A. and Rojas, A. and Chau, Y.Y. and Wagner, K.D. and Wagner, N. and Hastie, N.D. and Munoz-Chapuli, R. and Perez-Pomares, J.M.
Abstract:Recent reports suggest that mammalian embryonic coronary endothelium (CoE) originates from the sinus venosus and ventricular endocardium. However, the contribution of extracardiac cells to CoE is thought to be minor and nonsignificant for coronary formation. Using classic (Wt1(Cre)) and previously undescribed (G2-Gata4(Cre)) transgenic mouse models for the study of coronary vascular development, we show that extracardiac septum transversum/proepicardium (ST/PE)-derived endothelial cells are required for the formation of ventricular coronary arterio-venous vascular connections. Our results indicate that at least 20% of embryonic coronary arterial and capillary endothelial cells derive from the ST/PE compartment. Moreover, we show that conditional deletion of the ST/PE lineage-specific Wilms' tumor suppressor gene (Wt1) in the ST/PE ofG2-Gata4(Cre) mice and in the endothelium of Tie2(Cre) mice disrupts embryonic coronary transmural patterning, leading to embryonic death. Taken together, our results demonstrate that ST/PE-derived endothelial cells contribute significantly to and are required for proper coronary vascular morphogenesis.
Keywords:Coronary Endothelium, Septum Transversum, Proepicardium, Gata4, Wt1, Animals, Mice
Source:Proceedings of the National Academy of Sciences of the United States of America
ISSN:0027-8424
Publisher:National Academy of Sciences (U.S.A.)
Volume:113
Number:3
Page Range:656-661
Date:19 January 2016
Official Publication:https://doi.org/10.1073/pnas.1509834113
PubMed:View item in PubMed

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