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Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes

Item Type:Article
Title:Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes
Creators Name:Burridge, P.W. and Diecke, S. and Matsa, E. and Sharma, A. and Wu, H. and Wu, J.C.
Abstract:The generation of cardiomyocytes from human induced pluripotent stem cells (hiPSCs) provides a source of cells that accurately recapitulate the human cardiac pathophysiology. The application of these cells allows for modeling of cardiovascular diseases, providing a novel understanding of human disease mechanisms and assessment of therapies. Here, we describe a stepwise protocol developed in our laboratory for the generation of hiPSCs from patients with a specific disease phenotype, long-term hiPSC culture and cryopreservation, differentiation of hiPSCs to cardiomyocytes, and assessment of disease phenotypes. Our protocol combines a number of innovative tools that include a codon-optimized mini intronic plasmid (CoMiP), chemically defined culture conditions to achieve high efficiencies of reprogramming and differentiation, and calcium imaging for assessment of cardiomyocyte phenotypes. Thus, this protocol provides a complete guide to use a patient cohort on a testable cardiomyocyte platform for pharmacological drug assessment.
Keywords:Cardiomyocytes, Disease Modeling, Human Induced Pluripotent Stem Cells, Calcium Imaging
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Patient-Specific Induced Pluripotent Stem Cell Models
ISSN:1064-3745
ISBN:978-1-4939-3033-3
Publisher:Springer / Humana Press (U.S.A.)
Volume:1353
Page Range:119-130
Date:2016
Official Publication:https://doi.org/10.1007/7651_2015_196
PubMed:View item in PubMed

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