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Genome-wide polyadenylation maps reveal dynamic mRNA 3'-end formation in the failing human heart

Item Type:Article
Title:Genome-wide polyadenylation maps reveal dynamic mRNA 3'-end formation in the failing human heart
Creators Name:Creemers, E.E. and Bawazeer, A. and Ugalde, A.P. and van Deutekom, H.W.M. and van der Made, I. and de Groot, N.E. and Adriaens, M.E. and Cook, S.A. and Bezzina, C.R. and Hubner, N. and van der Velden, J. and Elkon, R. and Agami, R. and Pinto, Y.M.
Abstract:RATIONALE: Alternative cleavage and polyadenylation (APA) of mRNA represents a layer of gene regulation that to date has remained unexplored in the heart. This phenomenon may be very relevant, as the positioning of the polyA tail in mRNAs influences the length of the 3'UTR, a critical determinant of gene expression. OBJECTIVE: To investigate whether the 3'UTR length is regulated by APA in the human heart and whether this changes in the failing heart. METHODS AND RESULTS: We used 3'end RNA-sequencing (e3'-Seq) to directly measure global patterns of APA in healthy and failing human heart specimens. By monitoring polyadenylation profiles in these hearts, we identified disease-specific APA signatures in numerous genes. Interestingly, many of the genes with shortened 3'UTRs in heart failure were enriched for functional groups such as 'RNA binding', while genes with longer 3'UTRs were enriched for 'cytoskeletal organization' and 'actin binding'. RNA sequencing in a larger series of human hearts revealed that these APA candidates are often differentially expressed in failing hearts, with an inverse correlation between 3'UTR length and the level of gene expression. Protein levels of the APA regulator, Poly(A)-Binding Protein Nuclear 1 were substantially downregulated in failing hearts. CONCLUSIONS: We provide genome-wide, high-resolution polyadenylation maps of the human heart and show that the 3'end formation of mRNA is dynamic in heart failure, suggesting that APA-mediated 3'UTR length modulation represents an additional layer of gene regulation in failing hearts.
Keywords:3'UTR, Alternative Polyadenylation, Heart Failure, Gene Expression/Regulation, RNA Splicing Cardiomyopathy
Source:Circulation Research
ISSN:0009-7330
Publisher:American Heart Association (U.S.A.)
Volume:118
Number:3
Page Range:433-438
Date:5 February 2016
Official Publication:https://doi.org/10.1161/CIRCRESAHA.115.307082
PubMed:View item in PubMed

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