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Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9

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Official URL:https://doi.org/10.1186/s13059-015-0810-2
PubMed:View item in PubMed
Creators Name:Kuehn, R. and Chu, V.T.
Journal Title:Genome Biology
Journal Abbreviation:Genome Biol
Volume:16
Number:1
Page Range:244
Date:10 November 2015
Keywords:CRISPR-Cas Systems, Cell Line, Gene Targeting, Genome, Mutation, RNA Editing, Animals, Mammals
Abstract:The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step 'pop in & out' targeting approach facilitates this task.
ISSN:1465-6906
Publisher:BioMed Central (U.K.)
Item Type:Editorial

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