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Epoxides derived from dietary dihomo-gamma-linolenic acid induce germ cell death in C. elegans

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Item Type:Article
Title:Epoxides derived from dietary dihomo-gamma-linolenic acid induce germ cell death in C. elegans
Creators Name:Deline, M. and Keller, J. and Rothe, M. and Schunck, W.H. and Menzel, R. and Watts, J.L.
Abstract:Dietary fats are not created equally, slight differences in structure lead to crucial differences in function. Muticellular organisms use polyunsaturated fatty acid as substrates to produce potent signaling molecules crucial for many physiological processes, including reproduction. Here we explored the mechanism responsible for germ cell loss induced by dietary supplementation of dihomo-gamma-linolenic acid (DGLA, 20:3n-6) in the roundworm Caenorhabditis elegans. In this study we found that C. elegans CYP-33E2 activity produces a range of epoxy and hydroxy metabolites from dietary DGLA. Knockdown of cyp-33E2 suppressed the DGLA-induced sterility phenotype. Additionally, direct exposure of two specific DGLA-derived epoxy products, 8,9- and 14,15-epoxyeicosadienoic acids, produced germ cell abnormalities in the C. elegans gonad. We propose that sterility is mediated by the production of toxic DGLA-derived epoxides that trigger germ cell destruction. These studies are the first to establish a biological activity for a CYP-produced metabolite of DGLA.
Keywords:8,11,14-Eicosatrienoic Acid, Caenorhabditis Elegans Proteins, Cell Death, Cholesterol 7-{alpha}-Hydroxylase, Diet, Dietary Fats, Epoxy Compounds, Germ Cells, Animals, Caenorhabditis Elegans
Source:Scientific Reports
ISSN:2045-2322
Publisher:Nature Publishing Group (U.K.)
Volume:5
Page Range:15417
Date:21 October 2015
Official Publication:https://doi.org/10.1038/srep15417
PubMed:View item in PubMed

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