Helmholtz Gemeinschaft


Genome editing in mice using TALE nucleases

Item Type:Article
Title:Genome editing in mice using TALE nucleases
Creators Name:Wefers, B. and Brandl, C. and Ortiz, O. and Wurst, W. and Kühn, R.
Abstract:Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
Keywords:Knock-in, Knockout, One-cell Embryo, Gene Targeting, TALEN, Mouse, Animals, Mice
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:TALENs : methods and protocols
Publisher:Springer / Humana Press
Page Range:229-243
Number of Pages:274
Official Publication:https://doi.org/10.1007/978-1-4939-2932-0_17
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library