Imaging protein interactions by FRET microscopy: FLIM measurements

Item Type:	Article
Title:	Imaging protein interactions by FRET microscopy: FLIM measurements
Creators Name:	Verveer, P.J. and Rocks, O. and Harpur, A.G. and Bastiaens, P.I.H.
Abstract:	This image acquisition protocol is a basic plan for taking a fluorescence lifetime imaging (FLIM) series. FLIM makes live-cell FRET measurements based only on donor fluorescence more feasible, because lifetimes are independent of probe concentration and light path length. The former is not easy to determine in cells, and the latter means that cell shape is not a factor.
Source:	Cold Spring Harbor Protocols
ISSN:	1559-6095
Publisher:	Cold Spring Harbor Laboratory Press
Volume:	2006
Number:	6
Date:	1 November 2006
Official Publication:	https://doi.org/10.1101/pdb.prot4599
PubMed:	View item in PubMed

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