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Role of CYP eicosanoids in the regulation of pharyngeal pumping and food uptake in Caenorhabditis elegans

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Item Type:Article
Title:Role of CYP eicosanoids in the regulation of pharyngeal pumping and food uptake in Caenorhabditis elegans
Creators Name:Zhou, Y. and Falck, J.R. and Rothe, M. and Schunck, W.H. and Menzel, R.
Abstract:Cytochrome P450 (CYP)-dependent eicosanoids comprise epoxy- and hydroxy-metabolites of long-chain PUFAs (LC-PUFAs). In mammals, CYP eicosanoids contribute to the regulation of cardiovascular and renal function. Caenorhabditis elegans produces a large set of CYP eicosanoids; however, their role in worm's physiology is widely unknown. Mutant strains deficient in LC-PUFA/eicosanoid biosynthesis displayed reduced pharyngeal pumping frequencies. This impairment was rescued by long-term eicosapentaenoic and/or arachidonic acid supplementation, but not with a nonmetabolizable LC-PUFA analog. Short-term treatment with 17,18-epoxyeicosatetraenoic acid (17,18-EEQ), the most abundant CYP eicosanoid in C. elegans, was as effective as long-term LC-PUFA supplementation in the mutant strains. In contrast, 20-HETE caused decreased pumping frequencies. The opposite effects of 17,18-EEQ and 20-HETE were mirrored by the actions of neurohormones. 17,18-EEQ mimicked the stimulating effect of serotonin when added to starved worms, whereas 20-HETE shared the inhibitory effect of octopamine in the presence of abundant food. In wild-type worms, serotonin increased free 17,18-EEQ levels, whereas octopamine selectively induced the synthesis of hydroxy-metabolites. These results suggest that CYP eicosanoids may serve as second messengers in the regulation of pharyngeal pumping and food uptake in C. elegans.
Keywords:Cytochrome P45, Fatty Acid, Omega-3 Fatty Acids, Lipidomics, Pharynx, 17,18-EEQ, Animals, Caenorhabditis elegans
Source:Journal of Lipid Research
ISSN:0022-2275
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:56
Number:11
Page Range:2110-2123
Date:November 2015
Official Publication:https://doi.org/10.1194/jlr.M061887
PubMed:View item in PubMed

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