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| Item Type: | Article |
|---|---|
| Title: | Chamber identity programs drive early functional partitioning of the heart |
| Creators Name: | Mosimann, C. and Panáková, D. and Werdich, A.A. and Musso, G. and Burger, A. and Lawson, K.L. and Carr, L.A. and Nevis, K.R. and Sabeh, M.K and Zhou, Y. and Davidson, A.J. and DiBiase, A. and Burns, C.E. and Burns, C.G. and MacRae, C.A. and Zon, L.I. |
| Abstract: | The vertebrate heart muscle (myocardium) develops from the first heart field (FHF) and expands by adding second heart field (SHF) cells. While both lineages exist already in teleosts, the primordial contributions of FHF and SHF to heart structure and function remain incompletely understood. Here we delineate the functional contribution of the FHF and SHF to the zebrafish heart using the cis-regulatory elements of the draculin (drl) gene. The drl reporters initially delineate the lateral plate mesoderm, including heart progenitors. Subsequent myocardial drl reporter expression restricts to FHF descendants. We harnessed this unique feature to uncover that loss of tbx5a and pitx2 affect relative FHF versus SHF contributions to the heart. High-resolution physiology reveals distinctive electrical properties of each heart field territory that define a functional boundary within the single zebrafish ventricle. Our data establish that the transcriptional program driving cardiac septation regulates physiologic ventricle partitioning, which successively provides mechanical advantages of sequential contraction. |
| Keywords: | Cadherins, Cardiac Myocytes, Developmental Gene Expression Regulation, Genetically Modified Animals, Heart, Heart Atria, Heart Ventricles, LIM Domain Proteins, Latent TGF-{beta} Binding Proteins, Mesoderm, Myocardium, Myosin Light Chains, Nonmammalian Embryo, Salivary Proteins and Peptides, T-Box Domain Proteins, Transcription Factors, Transcriptional Regulatory Elements, Zebrafish Proteins, Animals, Zebrafish |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 6 |
| Page Range: | 8146 |
| Date: | 26 August 2015 |
| Official Publication: | https://doi.org/10.1038/ncomms9146 |
| PubMed: | View item in PubMed |
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