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Extensive allele-specific translational regulation in hybrid mice

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Item Type:Article
Title:Extensive allele-specific translational regulation in hybrid mice
Creators Name:Hou, J. and Wang, X. and McShane, E. and Zauber, H. and Sun, W. and Selbach, M. and Chen, W.
Abstract:Translational regulation is mediated through the interaction between diffusible trans-factors and cis-elements residing within mRNA transcripts. In contrast to extensively studied transcriptional regulation, cis-regulation on translation remains underexplored. Using deep sequencing-based transcriptome and polysome profiling, we globally profiled allele-specific translational efficiency for the first time in an F1 hybrid mouse. Out of 7,156 genes with reliable quantification of both alleles, we found 1,008 (14.1%) exhibiting significant allelic divergence in translational efficiency. Systematic analysis of sequence features of the genes with biased allelic translation revealed that local RNA secondary structure surrounding the start codon and proximal out-of-frame upstream AUGs could affect translational efficiency. Finally, we observed that the cis-effect was quantitatively comparable between transcriptional and translational regulation. Such effects in the two regulatory processes were more frequently compensatory, suggesting that the regulation at the two levels could be coordinated in maintaining robustness of protein expression.
Keywords:Allele‐Specific Gene Expression, cis‐Elements, Translational Regulation, Animals, Mice
Source:Molecular Systems Biology
ISSN:1744-4292
Publisher:EMBO Press
Volume:11
Number:8
Page Range:825
Date:7 August 2015
Official Publication:https://doi.org/10.15252/msb.156240
PubMed:View item in PubMed

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