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Small molecule screening in zebrafish: swimming in potential drug therapies

Item Type:Article
Title:Small molecule screening in zebrafish: swimming in potential drug therapies
Creators Name:Tamplin, O.J. and White, R.M. and Jing, L. and Kaufman, C.K. and Lacadie, S.A. and Li, P. and Taylor, A.M. and Zon, L.I.
Abstract:Phenotype-driven chemical genetic screens in zebrafish have become a proven approach for both dissection of developmental mechanisms and discovery of potential therapeutics. A library of small molecules can be arrayed into multiwell plates containing zebrafish embryos. The embryo becomes a whole organism in vivo bioassay that can produce a phenotype upon treatment. Screens have been performed that are based simply on the morphology of the embryo. Other screens have scored complex phenotypes using whole mount in situ hybridization, fluorescent transgenic reporters, and even tracking of embryo movement. The availability of many well-characterized zebrafish mutants has also enabled the discovery of chemical suppressors of genetic phenotypes. Importantly, the application of chemical libraries that already contain FDA-approved drugs has allowed the rapid translation of hits from zebrafish chemical screens to clinical trials.
Keywords:Genetic Testing, Nonmammalian Embryo, Phenotype, Small Molecule Libraries, Animals, Zebrafish
Source:Wiley Interdisciplinary Reviews: Developmental Biology
Page Range:459-468
Date:May 2012
Official Publication:https://doi.org/10.1002/wdev.37
PubMed:View item in PubMed

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