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miR-184 regulates pancreatic β-cell function according to glucose metabolism

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Item Type:Article
Title:miR-184 regulates pancreatic β-cell function according to glucose metabolism
Creators Name:Tattikota, S.G. and Rathjen, T. and Hausser, J. and Khedkar, A. and Kabra, U.D. and Pandey, V.K. and Sury, M.D. and Wessels, H.H. and Mollet, I.G. and Eliasson, L. and Selbach, M. and Zinzen, R.P. and Zavolan, M. and Kadener, S. and Tschöp, M. and Jastroch, M. and Friedländer, M.R. and Poy, M.N.
Abstract:In response to fasting or hyperglycemia, the pancreatic {beta}-cell alters its output of secreted insulin; however the pathways governing this adaptive response are not entirely established. While the precise role of microRNAs (miRNAs) is also unclear, a recurring theme emphasizes their function in cellular stress responses. We recently showed that miR-184, an abundant miRNA in the {beta}-cell, regulates compensatory proliferation and secretion during insulin resistance. Consistent with previous studies showing miR-184 suppresses insulin release, expression of this miRNA was increased in islets after fasting, demonstrating an active role in the {beta}-cell as glucose levels lower and the insulin demand ceases. Additionally, miR-184 was negatively regulated upon administration of a sucrose-rich diet in Drosophila demonstrating strong conservation of this pathway through evolution. Furthermore, miR-184 and its target Argonaute2 (Ago2) remained inversely correlated as concentrations of extracellular glucose increased, underlining a functional relationship between this miRNA and its targets. Lastly, restoration of Ago2 in the presence of miR-184 rescued suppression of miR-375-targeted genes suggesting these genes act in a coordinated manner during changes in the metabolic context. Together, these results highlight the adaptive role of miR-184 according to glucose metabolism and suggest the regulatory role of this miRNA in energy homeostasis is highly conserved.
Keywords:Glucose Metabolism, microRNA (miRNA), beta-Cell, Argonaute, Pancreatic Islet, Insulin, Insulin Secretion, microRNA Mechanism, Animals, Mice
Source:Journal of Biological Chemistry
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:20284-20294
Date:14 August 2015
Official Publication:https://doi.org/10.1074/jbc.M115.658625
PubMed:View item in PubMed

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