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Restoration of uneven illumination in light sheet microscopy images

Item Type:Article
Title:Restoration of uneven illumination in light sheet microscopy images
Creators Name:Uddin, M.S. and Lee, H.K. and Preibisch, S. and Tomancak, P.
Abstract:Light microscopy images suffer from poor contrast due to light absorption and scattering by the media. The resulting decay in contrast varies exponentially across the image along the incident light path. Classical space invariant deconvolution approaches, while very effective in deblurring, are not designed for the restoration of uneven illumination in microscopy images. In this article, we present a modified radiative transfer theory approach to solve the contrast degradation problem of light sheet microscopy (LSM) images. We confirmed the effectiveness of our approach through simulation as well as real LSM images.
Keywords:Digital Image Processing, Image Reconstruction-Restoration, Microscopy, Mathematical Methods in Physics
Source:Microscopy and Microanalysis
ISSN:1431-9276
Publisher:Cambridge University Press (U.K.)
Volume:17
Number:4
Page Range:607-613
Date:August 2011
Official Publication:https://doi.org/10.1017/S1431927611000262
PubMed:View item in PubMed

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