Gene gun Her2/neu DNA vaccination: evaluation of vaccine efficacy in a syngeneic Her2/neu mouse tumor model

Item Type:	Article
Title:	Gene gun Her2/neu DNA vaccination: evaluation of vaccine efficacy in a syngeneic Her2/neu mouse tumor model
Creators Name:	Nguyen-Hoai, T. and Pezzutto, A. and Westermann, J.
Abstract:	Genetic vaccination using naked plasmid DNA is an immunization strategy both against infectious diseases and cancer. In order to improve the efficacy of DNA vaccines, particularly in large animals and humans, different strategies have been pursued. These vaccination strategies are based on different application routes, schedules, and coexpression of immunomodulatory molecules as adjuvants. Our mouse tumor model offers the possibility to investigate Her2/neu DNA vaccines in different settings, i.e., intramuscular or intradermal application with or without coexpression of adjuvants. Protection from tumor growth in tumor challenge experiments and both T cell and humoral immune responses against Her2/neu peptides are used as surrogate parameters for vaccine efficacy.
Keywords:	Her2/neu+ Mouse Tumor Model, DNA Vaccination, Gene Gun Immunization, Her2/neu-Specific Immune Responses, Animals, Mice
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	Gene therapy of solid cancers : methods and protocols
ISSN:	1064-3745
ISBN:	978-1-4939-2726-5
Publisher:	Springer / Humana Press
Volume:	1317
Page Range:	17-37
Date:	2015
Official Publication:	https://doi.org/10.1007/978-1-4939-2727-2_2
PubMed:	View item in PubMed

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