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Oncoleaking: use of the pore-forming clostridium perfringens enterotoxin (CPE) for suicide gene therapy

Item Type:Article
Title:Oncoleaking: use of the pore-forming clostridium perfringens enterotoxin (CPE) for suicide gene therapy
Creators Name:Pahle, J. and Aumann, J. and Kobelt, D. and Walther, W.
Abstract:Suicide gene therapy has been shown to be very efficient in tumor eradication. Numerous suicide genes were tested in vitro and in vivo demonstrating their therapeutic potential in clinical trials. Apart from this, still growing efforts are made to generate more targeted and more effective suicide gene systems for cancer gene therapy. In this regard bacterial toxins are an alternative, which add to the broad spectrum of different suicide strategies. In this context, the claudin-targeted bacterial Clostridium perfringens enterotoxin (CPE) is an attractive new type of suicide oncoleaking gene, which as pore-forming protein exerts specific and rapid toxicity towards claudin-3- and -4-overexpressing cancers. In this chapter we describe the generation and use of CPE-expressing vectors for the effective tumor cell killing as novel suicide gene approach particularly for treatment of therapy refractory tumors.
Keywords:Clostridium Perfringens Enterotoxin, Pore-Forming Toxin, Bacterial Toxin, Suicide Gene Therapy, Claudin, Epithelial Tumor
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Gene therapy of solid cancers : methods and protocols
Publisher:Springer / Humana Press
Page Range:69-85
Official Publication:https://doi.org/10.1007/978-1-4939-2727-2_5
PubMed:View item in PubMed

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