Placental fractalkine mediates adhesion of THP-1 monocytes to villous trophoblast

Item Type:	Article
Title:	Placental fractalkine mediates adhesion of THP-1 monocytes to villous trophoblast
Creators Name:	Siwetz, M. and Sundl, M. and Kolb, D. and Hiden, U. and Herse, F. and Huppertz, B. and Gauster, M.
Abstract:	The chemokine fractalkine (CX3CL1) recently attracted increasing attention in the field of placenta research due to its dual nature, acting both as membrane-bound and soluble forms. While the membrane-bound form mediates flow-resistant adhesion of leukocytes to endothelial and epithelial cells via its corresponding receptor CX3CR1, the soluble form arises from metalloprotease-dependent shedding and bears chemoattractive activity for monocytes, natural killer cells and T cells. In human placenta, fractalkine is expressed at the apical microvillous plasma membrane of the syncytiotrophoblast, which may enable close physical contact with circulating maternal leukocytes. Based on these observations, we tested the hypothesis that fractalkine mediates adhesion of monocytes to the villous trophoblast. Forskolin-induced differentiation and syncytialization of the trophoblast cell line BeWo was accompanied with a substantial upregulation in fractalkine expression and led to increased adhesion of the monocyte cell line THP-1, which preferentially bound to syncytia. Blocking as well as silencing of the fractalkine receptor CX3CR1 proved involvement of the fractalkine/CX3CR1 system in adherence of THP-1 monocytes to villous trophoblast. Pre-incubation of THP-1 monocytes with human recombinant fractalkine as well as silencing of CX3CR1 expression in THP-1 monocytes significantly impaired their adherence to BeWo cells and primary term trophoblasts. The present study suggests fractalkine as another candidate among the panel of adhesion molecules enabling stable interaction between leukocytes and the syncytiotrophoblast.
Keywords:	Placental Fractalkine, Chemokine, Trophoblast, Monocyte, Adhesion
Source:	Histochemistry and Cell Biology
ISSN:	0948-6143
Publisher:	Springer
Volume:	143
Number:	6
Page Range:	565-574
Date:	June 2015
Additional Information:	Copyright © Springer-Verlag Berlin Heidelberg 2014
Official Publication:	https://doi.org/10.1007/s00418-014-1304-0
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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