Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Flow cytometric maturity score as a novel prognostic parameter in patients with acute myeloid leukemia

[img]
Preview
PDF (accepted manuscript (final draft)) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
424kB

Item Type:Article
Title:Flow cytometric maturity score as a novel prognostic parameter in patients with acute myeloid leukemia
Creators Name:Schneider, T. and Flörcken, A. and Singh, A. and Türkmen, S. and Burmeister, T. and Anagnostopoulos, I. and Pezzutto, A. and Dörken, B. and Westermann, J.
Abstract:The European LeukemiaNet (ELN) classification is widely accepted for risk stratification of patients with acute myeloid leukemia (AML). In order to establish immunophenotypic features that predict prognosis, the expression of single AML blast cell antigens has been evaluated with partly conflicting results; however, the influence of immunophenotypic blast maturity is largely unknown. In our study, 300 AML patients diagnosed at our institution between January 2003 and April 2012 were analyzed. A flow cytometric maturity score was developed in order to distinguish "mature" AML (AML-ma) from "immature" AML (AML-im) by quantitative expression levels of early progenitor cell antigens (CD34, CD117, and TdT). AML-ma showed significantly longer relapse-free survival (RFS) and overall survival (OS) than AML-im (p < 0.001). Interestingly, statistically significant differences in RFS and OS were maintained within the "intermediate-risk" group according to ELN (RFS, 7.0 years (AML-ma) vs. 3.3 years (AML-im); p = 0.002; OS, 5.1 years (AML-ma) vs. 3.0 years (AML-im); p = 0.022). Our novel flow cytometric score easily determines AML blast maturity and can predict clinical outcome. It remains to be clarified whether these results simply reflect an accumulation of favorable molecular phenotypes in the AML-ma subgroup or whether they rely on biological differences such as a higher proportion of leukemia stem cells and/or a higher degree of genetic instability within the AML-im subgroup.
Keywords:AML, Flow Cytometry, Maturity, Prognosis, Score
Source:Annals of Hematology
ISSN:0939-5555
Publisher:Springer (Germany)
Volume:94
Number:8
Page Range:1337-1345
Date:August 2015
Official Publication:https://doi.org/10.1007/s00277-015-2400-5
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library