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Photoswitchable fatty acids enable optical control of TRPV1

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Item Type:Article
Title:Photoswitchable fatty acids enable optical control of TRPV1
Creators Name:Frank, J.A. and Moroni, M. and Moshourab, R. and Sumser, M. and Lewin, G.R. and Trauner, D.
Abstract:Fatty acids (FAs) are not only essential components of cellular energy storage and structure, but play crucial roles in signalling. Here we present a toolkit of photoswitchable FA analogues (FAAzos) that incorporate an azobenzene photoswitch along the FA chain. By modifying the FAAzos to resemble capsaicin, we prepare a series of photolipids targeting the Vanilloid Receptor 1 (TRPV1), a non-selective cation channel known for its role in nociception. Several azo-capsaicin derivatives (AzCAs) emerge as photoswitchable agonists of TRPV1 that are relatively inactive in the dark and become active on irradiation with ultraviolet-A light. This effect can be rapidly reversed by irradiation with blue light and permits the robust optical control of dorsal root ganglion neurons and C-fibre nociceptors with precision timing and kinetics not available with any other technique. More generally, we expect that photolipids will find many applications in controlling biological pathways that rely on protein-lipid interactions.
Keywords:Azo Compounds, Bradykinin, Capsaicin, Fatty Acids, Spinal Ganglia, HEK293 Cells, In Vitro Techniques, Lidocaine, Lipid Metabolism, Photochemical Processes, Photochemistry, Serotonin, Spinal Ganglia, TRPV Cation Channels, Animals, Mice
Source:Nature Communications
Publisher:Nature Publishing Group
Page Range:7118
Date:21 May 2015
Official Publication:https://doi.org/10.1038/ncomms8118
PubMed:View item in PubMed

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