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Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain

Item Type:Article
Title:Arabidopsis KCBP interacts with AIR9 but stays in the cortical division zone throughout mitosis via its MyTH4-FERM domain
Creators Name:Buschmann, H. and Dols, J. and Kopischke, S. and Pena, E.J. and Andrade-Navarro, M.A. and Heinlein, M. and Szymanski, D.B. and Zachgo, S. and Doonan, J.H. and Lloyd, C.W.
Abstract:The preprophase band of microtubules performs the critical function of marking the plane of cell division. Although the preprophase band depolymerises at the onset of mitosis, the division plane is 'memorized' by a cortical division zone to which the phragmoplast is attracted during cytokinesis. Proteins have been discovered that are part of the molecular memory but little is known about how they contribute to phragmoplast guidance. Previously, we found that the microtubule-associated protein AIR9 is found in the cortical division zone at preprophase and returns during cell plate insertion but is absent from the cortex during the intervening mitosis. To identify new components of the preprophase memory we searched for protein interactors of AIR9. We detected the kinesin-like calmodulin binding protein, KCBP, which can be visualised at the predicted cortical site throughout division. A truncation study of KCBP indicates that its MyTH4-FERM domain is required for linking the motor domain to the cortex. These results suggest a mechanism by which minus end-directed KCBP helps guide the centrifugally expanding phragmoplast to the cortical division site.
Keywords:AIR9, Kinesin, Cell Division, MyTH4-FERM, Reprophase Band, Arabidopsis
Source:Journal of Cell Science
ISSN:0021-9533
Publisher:Company of Biologists (U.K.)
Volume:28
Number:11
Page Range:2033-2046
Date:1 June 2015
Official Publication:https://doi.org/10.1242/jcs.156570
PubMed:View item in PubMed

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