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Neural circular RNAs are derived from synaptic genes and regulated by development and plasticity

Official URL:https://doi.org/10.1038/nn.3975
PubMed:View item in PubMed
Creators Name:You, X. and Vlatkovic, I. and Babic, A. and Will, T. and Epstein, I. and Tushev, G. and Akbalik, G. and Wang, M. and Glock, C. and Quedenau, C. and Wang, X. and Hou, J. and Liu, H. and Sun, W. and Sambandan, S. and Chen, T. and Schuman, E.M. and Chen, W.
Journal Title:Nature Neuroscience
Journal Abbreviation:Nat Neurosci
Volume:18
Number:4
Page Range:603-610
Date:April 2015
Keywords:Brain, Dendrites, Hippocampus, In Situ Hybridization, Inbred C57BL Mice, Neuronal Plasticity, Neuropil, Patch-Clamp Techniques, RNA, RNA Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Sprague-Dawley Rats, Synapses, Animals, Mice, Rats
Abstract:Circular RNAs (circRNAs) have re-emerged as an interesting RNA species. Using deep RNA profiling in different mouse tissues, we observed that circRNAs were substantially enriched in brain and a disproportionate fraction of them were derived from host genes that encode synaptic proteins. Moreover, on the basis of separate profiling of the RNAs localized in neuronal cell bodies and neuropil, circRNAs were, on average, more enriched in the neuropil than their host gene mRNA isoforms. Using high-resolution in situ hybridization, we visualized circRNA punctae in the dendrites of neurons. Consistent with the idea that circRNAs might regulate synaptic function during development, many circRNAs changed their abundance abruptly at a time corresponding to synaptogenesis. In addition, following a homeostatic downscaling of neuronal activity many circRNAs exhibited substantial up- or downregulation. Together, our data indicate that brain circRNAs are positioned to respond to and regulate synaptic function.
ISSN:1097-6256
Publisher:Nature Publishing Group (U.S.A.)
Item Type:Article

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