Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells

Item Type:	Article
Title:	Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells
Creators Name:	Arner, E. and Daub, C.O. and Vitting-Seerup, K. and Andersson, R. and Lilje, B. and Drabløs, F. and Lennartsson, A. and Rönnerblad, M. and Hrydziuszko, O. and Vitezic, M. and Freeman, T.C. and Alhendi, A. and Arner, P. and Axton, R. and Baillie, J.K. and Beckhouse, A. and Bodega, B. and Briggs, J. and Brombacher, F. and Davis, M. and Detmar, M. and Ehrlund, A. and Endoh, M. and Eslami, A. and Fagiolini, M. and Fairbairn, L. and Faulkner, G.J. and Ferrai, C. and Fisher, M.E. and Forrester, L. and Goldowitz, D. and Guler, R. and Ha, T. and Hara, M. and Herlyn, M. and Ikawa, T. and Kai, C. and Kawamoto, H. and Khachigian, L. and Klinken, P.S. and Kojima, S. and Koseki, H. and Klein, S. and Mejhert, N. and Miyaguchi, K. and Mizuno, Y. and Morimoto, M. and Morris, K.J. and Mummery, C. and Nakachi, Y. and Ogishima, S. and Okada-Hatakeyama, M. and Okazaki, Y. and Orlando, V. and Ovchinnikov, D. and Passier, R. and Patrikakis, M. and Pombo, A. and Qin, X.Y. and Roy, S. and Sato, H. and Savvi, S. and Saxena, A. and Schwegmann, A. and Sugiyama, D. and Swoboda, R. and Tanaka, H. and Tomoiu, A. and Winteringham, L.N. and Wolvetang, E. and Yanagi-Mizuochi, C. and Yoneda, M. and Zabierowski, S. and Zhang, P. and Abugessaisa, I. and Bertin, N. and Diehl, A.D. and Fukuda, S. and Furuno, M. and Harshbarger, J. and Hasegawa, A. and Hori, F. and Ishikawa-Kato, S. and Ishizu, Y. and Itoh, M. and Kawashima, T. and Kojima, M. and Kondo, N. and Lizio, M. and Meehan, T.F. and Mungall, C.J. and Murata, M. and Nishiyori-Sueki, H. and Sahin, S. and Sato-Nagao, S. and Severin, J. and de Hoon, M.J. and Kawai, J. and Kasukawa, T. and Lassmann, T. and Suzuki, H. and Kawaji, H. and Summers, K.M. and Wells, C. and Hume, D.A. and Forrest, A.R.R. and Sandelin, A. and Carninci, P. and Hayashizaki, Y.
Abstract:	Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets of genes has not been previously studied en masse. Exploiting the fact that active promoters and enhancers are transcribed, we simultaneously measured their activity in 19 human and 14 mouse time courses covering a wide range of cell types and biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated the earliest responses. Binding sites for key lineage transcription factors were simultaneously overrepresented in enhancers and promoters active in each cellular system. Our data support a highly generalizable model in which enhancer transcription is the earliest event in successive waves of transcriptional change during cellular differentiation or activation.
Keywords:	Binding Sites, Cell Differentiation, Developmental Gene Expression Regulation, Genetic Enhancer Elements, Messenger RNA, Stem Cells, Transcription Factors, Genetic Transcription, Animals, Cattle, Dogs, Mice, Rats
Source:	Science
ISSN:	0036-8075
Publisher:	American Association for the Advancement of Science
Volume:	347
Number:	6225
Page Range:	1010-1014
Date:	27 February 2015
Additional Information:	Copyright © 2015, American Association for the Advancement of Science.
Official Publication:	https://doi.org/10.1126/science.1259418
External Fulltext:	View full text on PubMed Central
PubMed:	View item in PubMed

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