Experimental applications of TNF-reporter mice with far-red fluorescent label

Item Type:	Article
Title:	Experimental applications of TNF-reporter mice with far-red fluorescent label
Creators Name:	Shebzukhov, Y.V. and Kuchmiy, A.A. and Kruglov, A.A. and Zipp, F. and Siffrin, V. and Nedospasov, S.A.
Abstract:	This chapter provides protocols for in vitro and in vivo analysis of TNF-producing cells from a novel TNF reporter mouse. In these transgenic mice, genetic sequence encoding far-red reporter protein Katyushka (FRFPK) was placed under control of the same regulatory elements as TNF, thus providing the basis for detection, isolation, and visualization of TNF-producing cells.
Keywords:	TNF, Reporter Mice, Far-red Reporter Protein Katyushka, Flow Cytometry, Confocal Microscopy, Animals, Mice
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	The TNF Superfamily
ISSN:	1064-3745
ISBN:	978-1-4939-0668-0
Publisher:	Springer / Humana Press
Volume:	1155
Page Range:	151-162
Date:	10 April 2014
Official Publication:	https://doi.org/10.1007/978-1-4939-0669-7_13
PubMed:	View item in PubMed

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