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Experimental applications of TNF-reporter mice with far-red fluorescent label

Item Type:Article
Title:Experimental applications of TNF-reporter mice with far-red fluorescent label
Creators Name:Shebzukhov, Y.V. and Kuchmiy, A.A. and Kruglov, A.A. and Zipp, F. and Siffrin, V. and Nedospasov, S.A.
Abstract:This chapter provides protocols for in vitro and in vivo analysis of TNF-producing cells from a novel TNF reporter mouse. In these transgenic mice, genetic sequence encoding far-red reporter protein Katyushka (FRFPK) was placed under control of the same regulatory elements as TNF, thus providing the basis for detection, isolation, and visualization of TNF-producing cells.
Keywords:TNF, Reporter Mice, Far-red Reporter Protein Katyushka, Flow Cytometry, Confocal Microscopy, Animals, Mice
Source:Methods in Molecular Biology
Series Name: Methods in Molecular Biology
Title of Book:The TNF Superfamily
Publisher:Springer / Humana Press
Page Range:151-162
Date:10 April 2014
Official Publication:https://doi.org/10.1007/978-1-4939-0669-7_13
PubMed:View item in PubMed

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