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| Item Type: | Article |
|---|---|
| Title: | Protein kinase Ymr291w/Tda1 is essential for glucose signaling in Saccharomyces cerevisiae on the level of hexokinase isoenzyme ScHxk2 phosphorylation |
| Creators Name: | Kaps, S. and Kettner, K. and Migotti, R. and Kanashova, T. and Krause, U. and Roedel, G. and Dittmar, G. and Kriegel, T.M. |
| Abstract: | The enzyme ScHxk2 of Saccharomyces cerevisiae is a dual-function hexokinase that besides its catalytic role in glycolysis is involved in the transcriptional regulation of glucose-repressible genes. Relief from glucose repression is accompanied by the phosphorylation of the nuclear fraction of ScHxk2 at serine 15 and the translocation of the phosphoenzyme into the cytosol. Different studies suggest different serine/threonine protein kinases, Ymr291w/Tda1 or Snf1, to accomplish ScHxk2-S15 phosphorylation. The current paper provides evidence that Ymr291w/Tda1 is essential for that modification while protein kinases Ydr477w/Snf1, Ynl307c/Mck1, Yfr014c/Cmk1 and Ykl126w/Ypk1, which co-purified during Ymr291w/Tda1 tandem affinity purification, as well as protein kinases PKA and PKB homolog Sch9 are dispensable. Taking into account the detection of a significantly higher amount of the Ymr291w/Tda1 protein in cells grown in low-glucose media as compared to a high-glucose environment, Ymr291w/Tda1 is likely to contribute to glucose signaling in Saccharomyces cerevisiae on the level of ScHxk2-S15 phosphorylation in a situation of limited external glucose availability. The evolutionary conservation of amino acid residue serine 15 in yeast hexokinases and its phosphorylation is illustrated by the finding that YMR291W/TDA1 of Saccharomyces cerevisiae and the homologous KLLA0A09713 gene of Kluyveromyces lactis allow for cross-complementation of the respective protein kinase single-gene deletion strains. |
| Keywords: | Hexokinase, Phosphorylation, Protein Kinase A (Pka), Saccharomyces Cerevisiae, Signaling, Kluyveromyces Lactis, ScHxk2, Sch9, Snf1, Ymr291w/Tda1 |
| Source: | Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Volume: | 290 |
| Number: | 10 |
| Page Range: | 6243-6255 |
| Date: | 6 March 2015 |
| Official Publication: | https://doi.org/10.1074/jbc.M114.595074 |
| PubMed: | View item in PubMed |
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