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Sex dependent differences in renal angiotensinogen as an early marker of diabetic nephropathy

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Item Type:Article
Title:Sex dependent differences in renal angiotensinogen as an early marker of diabetic nephropathy
Creators Name:de Alencar Franco Costa, D. and Todiras, M. and Campos, L.A. and Cipolla-Neto, J. and Bader, M. and Baltatu, O.C.
Abstract:AIM: The renal renin-angiotensin system (RAS) has been implicated in the pathogenesis of diabetic nephropathy. The aim of this study was to investigate sex differences in renal renin-angiotensin system (RAS) and the roles of androgens in diabetes-associated renal injury. METHODS: Renal injury and fibrosis was studied in streptozotocin-induced diabetic rats by albuminuria and by gene expression of collagen-I and fibronectin. RAS was investigated by analyzing the plasma angiotensinogen (AOGEN) and renin activity (PRA) and their renal gene expression. Also, a group of diabetic rats was treated with the antiandrogen flutamide. RESULTS: Albuminuria was significantly lower in diabetic females than in males (1.2 [0.8-1.5] vs. 4.4 [2.2-6.1] mg/24h, data are median [IQR] values, p<0.05). Renal AOGEN mRNA levels were increased by diabetes in males (8.1 +/- 0.8% in diabetes vs 0.8 +/- 0.2% in control, p< 0.001) but not in females (1.0 +/- 0.1% in diabetes vs 0.8 +/- 0.1% in control, p>0.05), as were collagen-I and fibronectin mRNAs. Furthermore, AOGEN mRNA levels were strongly correlated with albuminuria (Spearman r = 0.64, 95%[CI] 0.36 to 0.81, p< 0.0001). Diabetes decreased PRA, renal renin mRNA and plasma AOGEN in both females and males. Antiandrogen treatment decreased albuminuria only in diabetic males without affecting the endocrine or renal RAS. CONCLUSIONS: These data indicate that renal but not hepatic AOGEN or renin is positively associated with diabetic albuminuria and contribute to the sex-dependent differences in renal injury. Androgens may contribute to albuminuria in male independently of the RAS.
Keywords:Diabetes, Renal Angiotensinogen, Albuminuria, Androgens, Sexual Dimorphism, Animals, Rats
Source:Acta Physiologica
ISSN:1748-1708
Publisher:Wiley-Blackwell (U.K.)
Volume:213
Number:3
Page Range:740-746
Date:March 2015
Official Publication:https://doi.org/10.1111/apha.12441
PubMed:View item in PubMed

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