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The subpopulation of microglia expressing functional muscarinic acetylcholine receptors expands in stroke and Alzheimer's disease

Item Type:Article
Title:The subpopulation of microglia expressing functional muscarinic acetylcholine receptors expands in stroke and Alzheimer's disease
Creators Name:Pannell, M. and Meier, M.A. and Szulzewsky, F. and Matyash, V. and Endres, M. and Kronenberg, G. and Prinz, V. and Waiczies, S. and Wolf, S.A. and Kettenmann, H.
Abstract:Microglia undergo a process of activation in pathology which is controlled by many factors including neurotransmitters. We found that a subpopulation (11 %) of freshly isolated adult microglia respond to the muscarinic acetylcholine receptor agonist carbachol with a Ca(2+) increase and a subpopulation of similar size (16 %) was observed by FACS analysis using an antibody against the M3 receptor subtype. The carbachol-sensitive population increased in microglia/brain macrophages isolated from tissue of mouse models for stroke (60 %) and Alzheimer's disease (25 %), but not for glioma and multiple sclerosis. Microglia cultured from adult and neonatal brain contained a carbachol-sensitive subpopulation (8 and 9 %), which was increased by treatment with interferon-{gamma} to around 60 %. This increase was sensitive to blockers of protein synthesis and correlated with an upregulation of the M3 receptor subtype and with an increased expression of MHC-I and MHC-II. Carbachol was a chemoattractant for microglia and decreased their phagocytic activity.
Keywords:Muscarinic Acetylcholine Receptors, Microglia, Brain Macrophages, Stroke, Alzheimer's Disease, Glioma, Multiple Sclerosis, Animals, Mice
Source:Brain Structure & Function
ISSN:1863-2653
Publisher:Springer (Germany)
Volume:221
Number:2
Page Range:1157-1172
Date:March 2016
Official Publication:https://doi.org/10.1007/s00429-014-0962-y
PubMed:View item in PubMed

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