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Dynamic regulation of the transcription initiation landscape at single nucleotide resolution during vertebrate embryogenesis

Item Type:Article
Title:Dynamic regulation of the transcription initiation landscape at single nucleotide resolution during vertebrate embryogenesis
Creators Name:Nepal, C. and Hadzhiev, Y. and Previti, C. and Haberle, V. and Li, N. and Takahashi, H. and Suzuki, A.M.M. and Sheng, Y. and Abdelhamid, R.F. and Anand, S. and Gehrig, J. and Akalin, A. and Kockx, C.E.M. and van der Sloot, A.A.J. and van Ijcken, W.F.J. and Armant, O. and Rastegar, S. and Watson, C. and Strähle, U. and Stupka, E. and Carninci, P. and Lenhard, B. and Müller, F.
Abstract:Spatiotemporal control of gene expression is central to animal development. Core promoters represent a previously unanticipated regulatory level by interacting with cis-regulatory elements and transcription initiation in different physiological and developmental contexts. Here, we provide a first and comprehensive description of the core promoter repertoire and its dynamic use during the development of a vertebrate embryo. By using cap analysis of gene expression (CAGE), we mapped transcription initiation events at single nucleotide resolution across 12 stages of zebrafish development. These CAGE-based transcriptome maps reveal genome-wide rules of core promoter usage, structure, and dynamics, key to understanding the control of gene regulation during vertebrate ontogeny. They revealed the existence of multiple classes of pervasive intra- and intergenic post-transcriptionally processed RNA products and their developmental dynamics. Among these RNAs, we report splice donor site-associated intronic RNA (sRNA) to be specific to genes of the splicing machinery. For the identification of conserved features, we compared the zebrafish data sets to the first CAGE promoter map of Tetraodon and the existing human CAGE data. We show that a number of features, such as promoter type, newly discovered promoter properties such as a specialized purine-rich initiator motif, as well as sRNAs and the genes in which they are detected, are conserved in mammalian and Tetraodon CAGE-defined promoter maps. The zebrafish developmental promoterome represents a powerful resource for studying developmental gene regulation and revealing promoter features shared across vertebrates.
Source:Genome Research
ISSN:1088-9051
Publisher:Cold Spring Harbor Laboratory Press (U.S.A.)
Volume:23
Number:11
Page Range:1938-1950
Date:November 2013
Official Publication:https://doi.org/10.1101/gr.153692.112
PubMed:View item in PubMed

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