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Two independent transcription initiation codes overlap on vertebrate core promoters

Item Type:Article
Title:Two independent transcription initiation codes overlap on vertebrate core promoters
Creators Name:Haberle, V. and Li, N. and Hadzhiev, Y. and Plessy, C. and Previti, C. and Nepal, C. and Gehrig, J. and Dong, X. and Akalin, A. and Suzuki, A.M. and van IJcken, W.F.J. and Armant, O. and Ferg, M. and Strähle, U. and Carninci, P. and Müller, F. and Lenhard, B.
Abstract:A core promoter is a stretch of DNA surrounding the transcription start site (TSS) that integrates regulatory inputs and recruits general transcription factors to initiate transcription. The nature and causative relationship of the DNA sequence and chromatin signals that govern the selection of most TSSs by RNA polymerase II remain unresolved. Maternal to zygotic transition represents the most marked change of the transcriptome repertoire in the vertebrate life cycle. Early embryonic development in zebrafish is characterized by a series of transcriptionally silent cell cycles regulated by inherited maternal gene products: zygotic genome activation commences at the tenth cell cycle, marking the mid-blastula transition. This transition provides a unique opportunity to study the rules of TSS selection and the hierarchy of events linking transcription initiation with key chromatin modifications. We analysed TSS usage during zebrafish early embryonic development at high resolution using cap analysis of gene expression, and determined the positions of H3K4me3-marked promoter-associated nucleosomes. Here we show that the transition from the maternal to zygotic transcriptome is characterized by a switch between two fundamentally different modes of defining transcription initiation, which drive the dynamic change of TSS usage and promoter shape. A maternal-specific TSS selection, which requires an A/T-rich (W-box) motif, is replaced with a zygotic TSS selection grammar characterized by broader patterns of dinucleotide enrichments, precisely aligned with the first downstream (+1) nucleosome. The developmental dynamics of the H3K4me3-marked nucleosomes reveal their DNA-sequence-associated positioning at promoters before zygotic transcription and subsequent transcription-independent adjustment to the final position downstream of the zygotic TSS. The two TSS-defining grammars coexist, often physically overlapping, in core promoters of constitutively expressed genes to enable their expression in the two regulatory environments. The dissection of overlapping core promoter determinants represents a framework for future studies of promoter structure and function across different regulatory contexts.
Keywords:Base Sequence, Developmental Gene Expression Regulation, Genetic Promoter Regions, Genetic Transcription Initiation, Histones, Methylation, Mothers, Nonmammalian Embryo, Nucleosomes, Transcription Initiation Site, Transcriptome, Zygote, Animals, Zebrafish
Source:Nature
ISSN:0028-0836
Publisher:Nature Publishing Group (U.K.)
Volume:507
Number:7492
Page Range:381-385
Date:20 March 2014
Official Publication:https://doi.org/10.1038/nature12974
PubMed:View item in PubMed

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