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Whole-mount in situ hybridization using DIG-labeled probes in planarian

Item Type:Article
Title:Whole-mount in situ hybridization using DIG-labeled probes in planarian
Creators Name:Rybak-Wolf, A. and Solana, J.
Abstract:In recent years freshwater flatworms (planarian) have become a powerful model for studies of regeneration and stem cell biology. Whole-mount in situ hybridization (WISH) and fluorescent in situ hybridization (FISH) are key and most commonly used techniques to determine and visualize gene expression patterns in planaria. Here, we present the established version of whole-mount in situ hybridization (WISH) and whole-mount fluorescence in situ hybridization (WFISH) protocol optimized over the last years by several labs from the rapidly growing planaria field and give an overview of recently introduced modifications which can be critical in the study of low abundant transcripts.
Keywords:Planarian, Schmidtea Mediterranea, Whole-Mount In Situ Hybridization (WISH), Fluorescent In Situ Hybridization (FISH), DIG-Labeled Probes, Animals, Planarians
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:In Situ Hybridization Protocols
ISSN:1064-3745
ISBN:978-1-4939-1458-6
Publisher:Springer / Humana Press (U.S.A.)
Volume:1211
Page Range:41-51
Date:2014
Official Publication:https://doi.org/10.1007/978-1-4939-1459-3_4
PubMed:View item in PubMed

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