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Elevated vascular level of ortho-tyrosine contributes to the impairment of insulin-induced arterial relaxation

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Item Type:Article
Title:Elevated vascular level of ortho-tyrosine contributes to the impairment of insulin-induced arterial relaxation
Creators Name:Szijártó, I.A. and Molnár, G.A. and Mikolás, E. and Fisi, V. and Cseh, J. and Laczy, B. and Kovács, T. and Böddi, K. and Takátsy, A. and Gollasch, M. and Koller, A. and Wittmann, I.
Abstract:Previous studies have shown that in diabetes mellitus, insulin-induced relaxation of arteries is impaired and the level of ortho-tyrosine (o-Tyr), an oxidized amino acid is increased. Thus, we hypothesized that elevated vascular level of o-Tyr contributes to the impairment of insulin-induced vascular relaxation. Rats were fed with o-Tyr for 4 weeks. Insulin-induced vasomotor responses of isolated femoral artery were studied using wire myography. Vascular o-Tyr content was measured by HPLC, whereas immunoblot analyses were preformed to detect eNOS phosphorylation. Sustained oral supplementation of rats with o-Tyr increased the content of o-Tyr in the arterial wall and significantly reduced the relaxations to insulin. Sustained supplementation of cultured endothelial cells with o-Tyr increased the incorporation of o-Tyr and mitigated eNOS Ser (1 177) phosphorylation to insulin. Increasing arterial wall o-Tyr level attenuates insulin-induced relaxation - at least in part - by decreasing eNOS activation. Elevated level of o-Tyr could be an underlying mechanism for vasomotor dysfunction in diabetes mellitus.
Keywords:Insulin, Vasomotor Function, ortho-Tyrosine, eNOS, Animals, Mice, Rats
Source:Hormone and Metabolic Research
ISSN:0018-5043
Publisher:Thieme (Germany)
Volume:46
Number:11
Page Range:749-752
Date:October 2014
Official Publication:https://doi.org/10.1055/s-0034-1387701
PubMed:View item in PubMed

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