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| Item Type: | Article |
|---|---|
| Title: | Simple derivation of transgene-free iPS cells by a dual recombinase approach |
| Creators Name: | Pertek, A., Meier, F., Irmler, M., Beckers, J., Skylaki, S., Endele, M., Wurst, W., Prakash, N. and Kühn, R. |
| Abstract: | Mammalian cells can be reprogrammed into induced pluripotent stem cells (iPSCs), a valuable tool for in vitro disease modeling and regenerative medicine. These applications demand for iPSCs devoid of reprogramming factor transgenes, but current procedures for the derivation of transgene-free iPSCs are inefficient and cumbersome. Here, we describe a new approach for the simple derivation of transgene-free iPSCs by the sequential use of two DNA recombinases, C31 Integrase and Cre, to control the genomic insertion and excision of a single, non-viral reprogramming vector. We show that such transgene-free iPSCs exhibit gene expression profiles and pluripotent developmental potential comparable to genuine, blastocyst-derived embryonic stem cells. As shown by a reporter iPSC line for the differentiation into midbrain dopaminergic neurons, the dual recombinase approach offers a simple and efficient way to derive transgene-free iPSCs for studying disease mechanisms and cell replacement therapies. |
| Keywords: | iPS cells, Reprogramming, phiC31 Integrase, Cre Recombinase, Pluripotency, Animals, Mice |
| Source: | Molecular Biotechnology |
| ISSN: | 1073-6085 |
| Publisher: | Springer / Humana Press |
| Volume: | 56 |
| Number: | 8 |
| Page Range: | 697-713 |
| Date: | August 2014 |
| Official Publication: | https://doi.org/10.1007/s12033-014-9748-y |
| PubMed: | View item in PubMed |
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