SecA defects are accompanied by dysregulation of MukB, DNA gyrase, chromosome partitioning and DNA superhelicity in Escherichia coli

Item Type:	Article
Title:	SecA defects are accompanied by dysregulation of MukB, DNA gyrase, chromosome partitioning and DNA superhelicity in Escherichia coli
Creators Name:	Adachi, S. and Murakawa, Y. and Hiraga, S.
Abstract:	Spatial regulation of nucleoids and chromosome-partitioning proteins is important for proper chromosome partitioning in Escherichia coli. However, the underlying molecular mechanisms are unknown. In the present work, we showed that mutation or chemical perturbation of secretory A (SecA), an ATPase component of the membrane protein translocation machinery, SecY, a component of the membrane protein translocation channel and acyl carrier protein P (AcpP), which binds to SecA and MukB, a functional homologue of structural maintenance of chromosomes protein (SMC), resulted in a defect in chromosome partitioning. We further showed that SecA is essential for proper positioning of the oriC DNA region, decatenation and maintenance of superhelicity of DNA. Genetic interaction studies revealed that the topological abnormality observed in the secA mutant was due to combined inhibitory effects of defects in MukB, DNA gyrase and Topo IV, suggesting a role for the membrane protein translocation machinery in chromosome partitioning and/or structural maintenance of chromosomes.
Keywords:	Adenosine Triphosphatases, Bacterial Chromosomes, Bacterial DNA, Bacterial Gene Expression Regulation, Bacterial Proteins, DNA Gyrase, Escherichia coli, Escherichia coli Proteins, Membrane Transport Proteins, Non-Histone Chromosomal Proteins, Superhelical DNA
Source:	Microbiology
ISSN:	1350-0872
Publisher:	Society for General Microbiology
Volume:	160
Number:	Pt 8
Page Range:	1648-1658
Date:	August 2014
Official Publication:	https://doi.org/10.1099/mic.0.077685-0
PubMed:	View item in PubMed

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