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Sustained release carrier for adenosine triphosphate as signaling molecule

Item Type:Article
Title:Sustained release carrier for adenosine triphosphate as signaling molecule
Creators Name:Wischke, C. and Weigel, J. and Bulavina, L. and Lendlein, A.
Abstract:Adenosine triphosphate (ATP) is a molecule with a fascinating variety of intracellular and extracellular biological functions that go far beyond energy metabolism. Due to its limited passive diffusion through biological membranes, controlled release systems may allow to interact with ATP-mediated extracellular processes. In this study, two release systems were explored to evaluate the capacity for either long-term or short-term release: (i) Poly[(rac-lactide)-co-glycolide] (PLGA) implant rods were capable of ATP release over days to weeks, depending on the PLGA molecular weight and end-group capping, but were also associated with partial hydrolytic degradation of ATP to ADP and AMP, but not adenosine. (ii) Thermosensitive methylcellulose hydrogels with a gelation occurring at body temperature allowed combining adjustable loading levels and the capacity for injection, with injection forces less than 50N even for small 27G needles. Finally, a first in vitro study illustrated purinergic-triggered response of primary murine microglia to ATP released from hydrogels, demonstrating the potential relevance for biomedical applications.
Keywords:Adenosine Triphosphate, Methyl Cellulose Hydrogel, PLGA Implant Rods, Controlled Release Carrier, Microglia Cells, Animals, Mice
Source:Journal of Controlled Release
ISSN:0168-3659
Publisher:Elsevier (The Netherlands)
Volume:195
Page Range:86-91
Date:10 December 2014
Official Publication:https://doi.org/10.1016/j.jconrel.2014.07.047
PubMed:View item in PubMed

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