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In vivo stable isotope labeling by amino acids in Drosophila melanogaster

Item Type:Article
Title:In vivo stable isotope labeling by amino acids in Drosophila melanogaster
Creators Name:Sury, M.D. and Chen, J.X. and Selbach, M.
Abstract:The fruit fly Drosophila melanogaster is one of the most widely used and well-studied model organisms in biology and therefore a promising tool for quantitative proteomics. Here, we describe a method to label D. melanogaster with stable isotope labeled amino acids in vivo. Feeding flies with heavy lysine labeled yeast cells leads to virtually complete heavy labeling already in the first filial generation. The approach is simple, fast, and cost-effective, which makes SILAC flies an attractive model system for the emerging field of in vivo quantitative proteomics.
Keywords:SILAC, In Vivo Labeling, Yeast, Protein Extraction, Animals, Drosophila melanogaster
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) : Methods and Protocols
ISSN:1064-3745
ISBN:978-1-4939-1141-7
Publisher:Springer / Humana Press (U.S.A.)
Volume:1188
Page Range:85-93
Date:8 July 2014
Official Publication:https://doi.org/10.1007/978-1-4939-1142-4_7
PubMed:View item in PubMed

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