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Comprehensive analysis of microRNA profiles in multiple sclerosis including next-generation sequencing

Item Type:Article
Title:Comprehensive analysis of microRNA profiles in multiple sclerosis including next-generation sequencing
Creators Name:Keller, A. and Leidinger, P. and Steinmeyer, F. and Stähler, C. and Franke, A. and Hemmrich-Stanisak, G. and Kappel, A. and Wright, I. and Dörr, J. and Paul, F. and Diem, R. and Tocariu-Krick, B. and Meder, B. and Backes, C. and Meese, E. and Ruprecht, K.
Abstract:BACKGROUND: MicroRNAs (miRNAs) are short, noncoding RNAs with gene regulatory functions whose expression profiles may serve as disease biomarkers. OBJECTIVE: The objective of this study was to perform a comprehensive analysis of miRNA expression profiles in blood of patients with a clinically isolated syndrome (CIS) or relapsing-remitting multiple sclerosis (RRMS) including next-generation sequencing (NGS). METHODS: miRNA expression was analyzed in whole blood samples from treatment-naive patients with CIS (n = 25) or RRMS (n = 25) and 50 healthy controls by NGS, microarray analysis, and quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: In patients with CIS/RRMS, NGS and microarray analysis identified 38 and eight significantly deregulated miRNAs, respectively. Three of these miRNAs were found to be significantly up- (hsa-miR-16-2-3p) or downregulated (hsa-miR-20a-5p, hsa-miR-7-1-3p) by both methods. Another five of the miRNAs significantly deregulated in the NGS screen showed the same direction of regulation in the microarray analysis. qRT-PCR confirmed the direction of regulation for all eight and was significant for three miRNAs. CONCLUSIONS: This study identifies a set of miRNAs deregulated in CIS/RRMS and reconfirms the previously reported underexpression of hsa-miR-20a-5p in MS. hsa-miR-20a-5p and the other validated miRNAs may represent promising candidates for future evaluation as biomarkers for MS and could be of relevance in the pathophysiology of this disease.
Keywords:Multiple Sclerosis, Clinically Isolated Syndrome, MicroRNAs, Biomarker, Next-Generation Sequencing, Microarray, Real-Time Polymerase Chain Reaction
Source:Multiple Sclerosis Journal
Publisher:Sage Publications
Page Range:295-303
Date:March 2014
Official Publication:https://doi.org/10.1177/1352458513496343
PubMed:View item in PubMed

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