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Paternal RNA contributions in the Caenorhabditis elegans zygote

Item Type:Article
Title:Paternal RNA contributions in the Caenorhabditis elegans zygote
Creators Name:Stoeckius, M. and Gruen, D. and Rajewsky, N.
Abstract:Development of the early embryo is thought to be mainly driven by maternal gene products and post-transcriptional gene regulation. Here, we used metabolic labeling to show that RNA can be transferred by sperm into the oocyte upon fertilization. To identify genes with paternal expression in the embryo, we performed crosses of males and females from divergent Caenorhabditis elegans strains. RNA sequencing of mRNAs and small RNAs in the 1-cell hybrid embryo revealed that about one hundred sixty paternal mRNAs are reproducibly expressed in the embryo and that about half of all assayed endogenous siRNAs and piRNAs are also of paternal origin. Together, our results suggest an unexplored paternal contribution to early development.
Keywords:Embryogenesis, Epigenetic Inheritance, Paternal RNA, Transgenerational Inheritance, Animals, Caenorhabditis elegans
Source:EMBO Journal
ISSN:0261-4189
Publisher:Nature Publishing Group (U.S.A.)
Volume:33
Number:16
Page Range:1740-1750
Date:18 August 2014
Official Publication:https://doi.org/10.15252/embj.201488117
PubMed:View item in PubMed

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