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| Item Type: | Article |
|---|---|
| Title: | Identification of the RNA recognition element of the RBPMS family of RNA-binding proteins and their transcriptome-wide mRNA targets |
| Creators Name: | Farazi, T.A. and Leonhardt, C.S. and Mukherjee, N. and Mihailovic, A. and Li, S. and Max, K.E.A. and Meyer, C. and Yamaji, M. and Cekan, P. and Jacobs, N.C. and Gerstberger, S. and Bognanni, C. and Larsson, E. and Ohler, U. and Tuschl, T. |
| Abstract: | Recent studies implicated the RNA-binding protein with multiple splicing (RBPMS) family of proteins in oocyte, retinal ganglion cell, heart, and gastrointestinal smooth muscle development. These RNA-binding proteins contain a single RNA recognition motif (RRM), and their targets and molecular function have not yet been identified. We defined transcriptome-wide RNA targets using photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in HEK293 cells, revealing exonic mature and intronic pre-mRNA binding sites, in agreement with the nuclear and cytoplasmic localization of the proteins. Computational and biochemical approaches defined the RNA recognition element (RRE) as a tandem CAC trinucleotide motif separated by a variable spacer region. Similar to other mRNA-binding proteins, RBPMS family of proteins relocalized to cytoplasmic stress granules under oxidative stress conditions suggestive of a support function for mRNA localization in large and/or multinucleated cells where it is preferentially expressed. |
| Keywords: | RNA-Binding Protein, PAR-CLIP, RNA Recognition Element, RBPMS, Animals, Mice |
| Source: | RNA |
| ISSN: | 1355-8382 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Volume: | 20 |
| Number: | 7 |
| Page Range: | 1090-1102 |
| Date: | July 2014 |
| Official Publication: | https://doi.org/10.1261/rna.045005.114 |
| PubMed: | View item in PubMed |
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