Design and generation of gene-targeting vectors

Item Type:	Article
Title:	Design and generation of gene-targeting vectors
Creators Name:	Wefers, B. and Wurst, W. and Kühn, R.
Abstract:	This unit provides an overview of the major types of mutant alleles that can be generated by gene targeting in ES cells. It presents the growing public resources of premade gene targeting vectors, modified ES cells, and mutant mice. General guidelines for the design of targeting vectors are followed by protocols for the construction of vectors to generate knockout (KO), conditional KO, and subtle mutant alleles.
Keywords:	Gene Targeting, Conditional KO Mice, Targeting Vector Design, Knockout Mice, EUCOMM/KOMP, Animals, Mice
Source:	Current Protocols in Mouse Biology
ISSN:	2161-2617
Publisher:	Wiley
Volume:	1
Page Range:	199-211
Date:	March 2011
Official Publication:	https://doi.org/10.1002/9780470942390.mo100179
PubMed:	View item in PubMed

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