Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Gene editing in one-cell embryos by zinc-finger and TAL nucleases

Item Type:Article
Title:Gene editing in one-cell embryos by zinc-finger and TAL nucleases
Creators Name:Wefers, B. and Meyer, M. and Hensler, S. and Panda, S. and Ortiz, O. and Wurst, W. and Kuehn, R.
Abstract:Gene targeting by sequence-specific nucleases in one-cell embryos provides an expedited mutagenesis approach in rodents. This technology has been recently established to create knockout and knockin mutants through sequence deletion or sequence insertion. This article provides protocols for the preparation and microinjection of nuclease mRNA and targeting vector DNA into fertilized mouse eggs. Furthermore, we provide guidelines for genotyping the desired mouse mutants.
Keywords:Pronucleus Injection, Gene Targeting, Mouse Mutant, Zinc-Finger Nuclease, TAL Nuclease, Homologous Recombination, Animals, Mice
Source:Current Protocols in Mouse Biology
ISSN:2161-2617
Publisher:Wiley (U.S.A.)
Volume:2
Page Range:347-364
Date:December 2012
Official Publication:https://doi.org/10.1002/9780470942390.mo120177

Repository Staff Only: item control page

Open Access
MDC Library