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MHC class I expression in mice lacking the proteasome subunit LMP-7

Item Type:Article
Title:MHC class I expression in mice lacking the proteasome subunit LMP-7
Creators Name:Fehling, H.J. and Swat, W. and Laplace, C. and Kühn, R. and Rajewsky, K. and Müller, U. and von Boehmer, H.
Abstract:Proteasomes degrade endogenous proteins. Two subunits, LMP-2 and LMP-7, are encoded in a region of the major histocompatibility complex (MHC) that is critical for class I-restricted antigen presentation. Mice with a targeted deletion of the gene encoding LMP-7 have reduced levels of MHC class I cell-surface expression and present the endogenous antigen HY inefficiently; addition of peptides to splenocytes deficient in LMP-7 restores wild-type class I expression levels. This demonstrates the involvement of LMP-7 in the MHC class I presentation pathway and suggests that LMP-7 functions as an integral part of the peptide supply machinery.
Keywords:ATP-Binding Cassette Transporters, Amino Acid Sequence, Antigen Presentation, Antigen-Presenting Cells, Base Sequence, Carrier Proteins, Cysteine Endopeptidases, Gene Deletion, H-2 Antigens, H-Y Antigen, Lymphocytes, Molecular Sequence Data, Multienzyme Complexes, Proteasome Endopeptidase Complex, Proteins, Animals, Mice
Source:Science
ISSN:0036-8075
Publisher:American Association for the Advancement of Science (U.S.A.)
Volume:265
Number:5176
Page Range:1234-1237
Date:26 August 1994
Official Publication:https://doi.org/10.1126/science.8066463
PubMed:View item in PubMed

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