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Generation and analysis of interleukin-4 deficient mice

Item Type:Article
Title:Generation and analysis of interleukin-4 deficient mice
Creators Name:Kühn, R. and Rajewsky, K. and Müller, W.
Abstract:Interleukin-4 (IL-4) promotes the growth and differentiation of many hematopoietic cells in vitro; in particular, it directs the immunoglobulin (Ig) class switch to IgG1 and IgE. Mice homozygous for a mutation that inactivates the IL-4 gene were generated to test the requirement for IL-4 in vivo. In the mutant mice T and B cell development was normal, but the serum levels of IgG1 and IgE were strongly reduced. The IgG1 dominance in a T cell-dependent immune response was lost, and IgE was not detectable upon nematode infection. Thus, some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo.
Keywords:Aging, Alleles, B-Lymphocytes, Chromosome Deletion, Concanavalin A, DNA, Interleukin-4, Lymph Nodes, Lymphocyte Activation, Restriction Mapping, Southern Blotting, Spleen, Thymus Gland, T-Lymphocytes, Animals, Inbred C57BL Mice, Mice, Mutant Strains Mice
Publisher:American Association for the Advancement of Science
Page Range:707-710
Date:1 November 1991
Official Publication:https://doi.org/10.1126/science.1948049
PubMed:View item in PubMed

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