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In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death

Item Type:Article
Title:In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death
Creators Name:Lam, K.P. and Kühn, R. and Rajewsky, K.
Abstract:Gene targeting experiments have demonstrated that the expression of immunoglobulin heavy chain in the pre-B cell receptor (pBCR) and of heavy and light chains in the B cell antigen receptor (BCR) marks checkpoints in early B cell development that the cells have to pass to survive. To investigate whether the persistence of mature B cells in the peripheral immune system also depends on BCR expression, we have generated a transgenic mouse in which the BCR can be inducibly ablated through V region gene deletion. Ablation leads to rapid death of mature B lymphocytes, which is preceded by down-regulation of MHC antigens and up-regulation of CD95 (Fas) and can be delayed by constitutive bcl-2 expression.
Keywords:Apoptosis, B-Lymphocytes, Cell Differentiation, Developmental Gene Expression Regulation, Flow Cytometry, Gene Deletion, Gene Targeting, Hematopoietic Stem Cells, Immunoglobulin Heavy Chains, Integrases, Oncogene Proteins, Phenotype, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Proto-Oncogene Proteins c-bcr, Surface Antigens, Transfection, Transgenic Mice, Viral Proteins, Animals, Mice
Source:Cell
ISSN:0092-8674
Publisher:Cell Press (U.S.A.)
Volume:90
Number:6
Page Range:1073-1083
Date:19 September 1997
Official Publication:https://doi.org/10.1016/S0092-8674(00)80373-6
PubMed:View item in PubMed

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