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Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation

Item Type:Article
Title:Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation
Creators Name:Eggan, K. and Rode, A. and Jentsch, I. and Samuel, C. and Hennek, T. and Tintrup, H. and Zevnik, B. and Erwin, J. and Loring, J. and Jackson-Grusby, L. and Speicher, M.R. and Kuehn, R. and Jaenisch, R.
Abstract:We have devised a general strategy for producing female mice from 39,X0 embryonic stem (ES) cells derived from male cell lines carrying a targeted mutation of interest. We show that the Y chromosome is lost in 2% of subclones from 40,XY ES cell lines, making the identification of targeted 39,X0 subclones a routine procedure. After gene targeting, male and female mice carrying the mutation can be generated by tetraploid embryo complementation from the 40,XY ES cell line and its 39,X0 derivatives. A single intercross then produces homozygous mutant offspring. Because this strategy avoids outcrossing and therefore segregation of mutant alleles introduced into the ES cells, the time and expense required for production of experimental mutant animals from a targeted ES cell clone are substantially reduced. Our data also indicate that ES cells have inherently unstable karyotypes, but this instability does not interfere with production of adult ES cell tetraploid mice.
Keywords:Alleles, Cell Line, Fluorescence In Situ Hybridization, Genetic Techniques, Genotype, Genotype, Homozygote, Karyotyping, Mammalian Embryo, Metaphase, Mutation, Organism Cloning, Ploidies, Sex Factors, Southern Blotting, Stem Cells, Time Factors, X Chromosome, Y Chromosome, Animals, Mice
Source:Nature Biotechnology
ISSN:1087-0156
Publisher:Nature Publishing Group (U.S.A.)
Volume:20
Number:5
Page Range:455-459
Date:May 2002
Official Publication:https://doi.org/10.1038/nbt0502-455
PubMed:View item in PubMed

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