PDF
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
372kB |
Item Type: | Article |
---|---|
Title: | Rapid generation of inducible mouse mutants |
Creators Name: | Seibler, J. and Zevnik, B. and Küter-Luks, B. and Andreas, S. and Kern, H. and Hennek, T. and Rode, A. and Heimann, C. and Faust, N. and Kauselmann, G. and Schoor, M. and Jaenisch, R. and Rajewsky, K. and Kühn, R. and Schwenk, F. |
Abstract: | We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol. |
Keywords: | Cell Line, Gene Expression, Genetic Engineering, Genetic Recombination, Integrases, Knockout Mice, Mammalian Embryo, Recombinant Fusion Proteins, Steroid Receptors, Transgenic Mice, Viral Proteins, Animals, Mice |
Source: | Nucleic Acids Research |
ISSN: | 0305-1048 |
Publisher: | Oxford University Press |
Volume: | 31 |
Number: | 4 |
Page Range: | e12 |
Date: | 15 February 2003 |
Official Publication: | https://doi.org/10.1093/nar/gng012 |
PubMed: | View item in PubMed |
Repository Staff Only: item control page