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Rapid generation of inducible mouse mutants

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Item Type:Article
Title:Rapid generation of inducible mouse mutants
Creators Name:Seibler, J. and Zevnik, B. and Küter-Luks, B. and Andreas, S. and Kern, H. and Hennek, T. and Rode, A. and Heimann, C. and Faust, N. and Kauselmann, G. and Schoor, M. and Jaenisch, R. and Rajewsky, K. and Kühn, R. and Schwenk, F.
Abstract:We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol.
Keywords:Cell Line, Gene Expression, Genetic Engineering, Genetic Recombination, Integrases, Knockout Mice, Mammalian Embryo, Recombinant Fusion Proteins, Steroid Receptors, Transgenic Mice, Viral Proteins, Animals, Mice
Source:Nucleic Acids Research
Publisher:Oxford University Press
Page Range:e12
Date:15 February 2003
Official Publication:https://doi.org/10.1093/nar/gng012
PubMed:View item in PubMed

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