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Novel caspase-suicide proteins for tamoxifen-inducible apoptosis

Item Type:Article
Title:Novel caspase-suicide proteins for tamoxifen-inducible apoptosis
Creators Name:Chu, Y. and Senghaas, N. and Koester, R.W. and Wurst, W. and Kuehn, R.
Abstract:Taking advantage of a mutant estrogen receptor ligand binding domain (ER(T2)), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase-ER(T2) fusion proteins become specifically activated by the synthetic ligand 4-OH- tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms.
Keywords:Caspase, Apoptosis, Tamoxifen, Cell Ablation, Estrogen Receptor, Animals, Mice, Zebrafish
Source:Genesis
ISSN:1526-954X
Publisher:Wiley (U.S.A.)
Volume:46
Number:10
Page Range:530-536
Date:October 2008
Official Publication:https://doi.org/10.1002/dvg.20426
PubMed:View item in PubMed

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